Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse IgG2a isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Amethyst Orange conjugated Mouse anti Human CD45 . All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer

Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Amethyst Orange conjugated Mouse IgG2a isotype control (MCA929AMO). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and Amethyst Orange conjugated Mouse anti Human CD45 (MCA87AMO). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer